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Sterile water contains no preservative, so once the stopper is punctured, the vial is susceptible to bacterial contamination

If a reconstituted vial accidentally freezes, thaw it slowly in the refrigerator (never at room temperature or under warm water), gently swirl to re-mix, and use it immediately rather than re-storing but expect measurably reduced activity compared to a never-frozen control

Haskell CF, Kennedy DO, Wesnes KA, Scholey AB (2005) Cognitive and mood improvements of caffeine in habitual consumers and habitual non-consumers of caffeine

If you add 1 mL instead, the concentration doubles to 5 mg/mL

Can factors like diet or metabolism change the 5-Amino-1MQ half life