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Occlusive layer if skin tolerates
M500KCAF0Y, Bio-Rad Laboratories) was used for cytokine profiling
Studies of lidocaine 1 % and 2 % diluted in dextrose retained 98 % potency for 90 days in polypropylene syringes, indicating no clinically relevant degradation in standard admixtures
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RAW 264.7 cells were cultured in complete Dulbeccos modified Eagle medium (DMEM, Gibco) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Avantor Seradigm), 2 mM L-glutamine (Gibco), 1 non-essential amino acids solution (Gibco) and 1 mM sodium pyruvate (Gibco) below 18 passages without exceeding 90% confluency
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