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The primer sequences used in this experiment are shown in Supplementary Table S3

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Briefly, cells are cultured with the STEMdiff TM definitive endoderm basal medium with supplements A and B for 24h followed by STEMdiff TM definitive endoderm basal medium with supplement B for 48h with daily medium change ( TM DE kit is based primarily on human activin A and Wnt3a ( Hepatoblast specification For small molecule hepatoblast specification, DE cells were washed with DPBS / and knockout DMEM (Thermo Fischer Scientific) and sequentially cultured with hepatoblast specification medium without passaging