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7 mm, volume 3 ml, Sigma), concentrated by vacuum centrifugation and reconstituted in 40 l of 0.1% (v/v) formic acid

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The calculated activation energies (G in kcal mol -1 ) for the abstraction the C2 proton of 7 to produce the cyanidin enolate ( 8-enolate) were found to be almost identical for the wild-type and the C13S variant, in agreement with their similar dehydratase activity measured experimentally

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Panthenol enhances hydration, calms skin, and supports the skin barrier