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The pelleted crypts were resuspended in 25 l/well growth factor reduced Matrigel (Corning, New York, USA)

Even in a standard refrigerator at 2-8C, the powder holds its potency for several months

SURMOUNT-3 added intensive lifestyle and reached 26.6%

Clinical Insights: Creatine in Calorie Deficits While most creatine research focuses on athletes, several trials demonstrate efficacy in lean mass preservation when calories are restricted: A meta-analysis in Nutr Metab showed that subjects in a 20-30% calorie deficit who took creatine maintained up to 60% more lean mass than non-supplemented controls over 8 weeks

Int J Cardiol (2002) 85:1519