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The total DNA concentration was adjusted to 30 ng L 1 ( Following total DNA extraction, the fungal ITS region was amplified using the eukaryotic primers ITS1F primer (5-CTTGGTCATTTAGAGGAAGTAA-3) and ITS2R (5-GCTGCGTTCTTCATCGATGC-3) ( Data Processing Single-end reads were generated (i.e., 400 bp/600 bp for fungi and 1,000 bp/1,200 bp for bacteria), followed by qualitative filtering and merging of raw reads by Cutadapt version 1.9.1

In the evenings I sometimes forgot to take off my nicotine patch, which gave me nightmares all night, he said

Sequence data were analyzed using CLC Genomic Workbench 9.5 (QIAGEN, Hilden, Germany) as described 19

We dont know who it was or when it was purchased, Walsh said