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found that acetaminophen causes concentration-dependent neuronal death in vitro at concentrations that are reached in human plasma during acetaminophen overdose, and are reached in the cerebrospinal fluid of rats for 3 h following doses that are below those required to induce acute hepatic failure in rats

After coating, each well was then incubated with biotin-BPC 157 and the first antibody, goat-anti-human-IgG-Fc-biotin (Abcam), followed by the second antibody, streptavidin-HRP

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