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Data-dependent MS2 fragmentation for compound identification and annotation was performed via the AquireX workflow (Thermo Scientific), which comprised three deep scans with MS1 resolution at 60,000 and MS2 resolution at 15,000

In fact, the clinical diagnosis of PD requires the presence of bradykinesia accompanied by at least one other motor symptom, such as resting tremor, muscle rigidity, or postural instability