The vapors are then rapidly cooled, triggering aerosol formation
I say it for the same reason they put warnings on packages of cigarettes, and fighting Chael Sonnen may be hazardous to one's health
Stephen Wilson, an assistant professor of psychology at the university, in a press release
Briefly, dpHBEC samples were harvested by the addition of 250 L of RIPA lysis buffer (50 mM tris-base, pH 8.0, 150 mM NaCl, 400 uM EDTA, 10% glycerol, 1% Triton-X, 0.1% SDS, 0.1% sodium deoxycholate, with 1X protease inhibitor (Sigma, #11697498001) and phosphatase inhibitor (Sigma, #04906837001) cocktails) to each insert, scraping with a wide-bore P200 pipette tip (United States Scientific, #10118410), and transfer to an Eppendorf tube prior to incubation on ice for 20 min
That is also why I strongly advocate putting down the cigar and calling it a day exactly when youre ready