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EMD Serono discontinued the product and its FDA approval was withdrawn due to difficulties in the manufacturing and marketing processes unrelated to its safety or effectiveness [4, 6]

For monoamines quantification, the mobile phase is a mixed solution consisting of OSA (1.7 mM), NaH 2 PO 4 2H 2 O (90.0 mM), C 6 H 8 O 7 H 2 O (50.0 mM), EDTA2Na (50.0 M) and 5% acetonitrile with the flow rate of 0.2 ml min 1

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2A and B, osthole is not cytoxic to PASMCs at 10 5 M to 10 9 M, and 10 6 M and 10 7 M osthole have capability to inhibit PASMCs proliferation induced with PDGF-BB, and were chosen for subsequent experiments