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The SOD defense by Cu/Zn-SOD, Fe/Mn-SOD, and extracellular SOD, is generally achieved by catalase or peroxidases, such as the GPxs, which use reduced glutathione (GSH) as electron donor

Protein concentrations were determined using the BCA protein assay kit (Beyotime, LTD Inc., Shanghai, China) according to the manufactorys instructions

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However, as with any emerging field, there are currently several different methods for collection, processing, storage, and calculation of summary measures of plasma glutathione metabolism, within and between preclinical and clinical research