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The optimal dosage and frequency are determined through consultation with healthcare professionals, who consider factors such as the underlying cause of deficiency, individual health status, and response to treatment

Target the outer thigh rather than the front, as the outer surface typically maintains more subcutaneous fat even in lean individuals

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Visualization and quantitative densitometry analysis were performed with Molecular Imager Gel Doc XR System v5.2.1 (Bio-Rad Laboratories, Hercules, CA, USA)