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Thick cross-sections at the mid-diaphysis were prepared using a diamond-embedded wire saw (Histosaw, Delaware Diamond Knives, Wilmington, DE, USA) and ground to a final thickness of 3040 m for periosteal and endosteal bone formation measurements

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The final PubMed search was completed in February 2025

The thickness should be asses sed at five distinct points (four corners and one in the centre)

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