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Z-stack images were taken on a Leica TCS SP8-STED using a HC PL APO 100x/1.40 CS2 objective with laser intensities adjusted to avoid signal saturation

Assay of caspase-3 activity GSH depleted HepG2 cells with or without NAC (210 6 cells/well) were treated with different concentrations of ASA as described above and the caspase-3 substrate, DEVD-pNA was used to assay the protease activity colorimetrically as described in the vendor's protocol (R & D Systems)

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Association of glucagon-like peptide-1 receptor-targeted imaging probe with in vivo glucagon-like peptide-1 receptor agonist glucose-lowering effects

Subsequently, 3.5 L of each sample was applied to a lacey grid that had been glow-discharged with a Leica ACE600 (Leica, Vienna, AT) coating unit within a humidity-controlled chamber of a Leica GP2 plunge-freezer