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Peripheral blood mononuclear cell (PBMC) was isolated with density-gradient centrifugation, then washed twice with ice-cold PBS and lysed in fresh lysis buffer consisting of 0.1M tetraethylammonium bromide (TEAB) (Thermo Fisher Scientific, MA, USA), 0.5% sodium dodecyl sulfate (SDS) (ST628, Beyotime, China), and 1X HALT protease and phosphatase inhibitor cocktail (78420, Thermo Fisher Scientific, MA, USA) (200L) for 10 min

Parasite Immunol

These misfolded alpha-synuclein aggregates propagate between neurons

Rahim, N

Injecting too superficially, failing to rotate sites adequately, or not allowing alcohol to dry completely before injection all contribute to local reactions