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For LC-MS analysis, samples were thawed on ice-water bath, then 40 L of whole blood was injected into 450 L cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35

Kozlowski P, Raj D, Liu J, Lam C, Yung AC, Tetzlaff W

A carefully planned diet with an appropriate intake of antioxidant-rich food and physical activity might protect against many pathologies, particularly diabetes, obesity and cardiovascular complications [[15], [16], [17]]

Stool samples were then rated according to the Bristol Stool Scale (BSS) ( 260 /OD 280 ratio of all samples was 1.80 (demonstrating DNA purity)