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Challenges in Tag Affinity Chromatography A survey of researchers who use tagged protein purification identified the top three challenges they commonly faced: Low protein purity Low recovery Long and tedious procedures for tag removal While His-tag purification has minimal impact on protein structure and purification, proteins can exhibit non-specific binding
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*Correspondence: Antonio Masi, [email protected] This article was submitted to Plant Proteomics, a section of the journal Frontiers in Plant Science Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers
Raghu G, Berk M, Campochiaro PA, Jaeschke H, Marenzi G, Richeldi L, Wen F, Nicoletti F, Calverley P (2020) The multifaceted therapeutic role of N-Acetylcysteine (NAC) in disorders characterized by oxidative stress
