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The breakdown of its various flavors is as follows: blueberry raspberry at 0.9%, lime romantic at 0.8%, peach ice at 0.8%, aloe mango melon ice at 0.6%, cool mint at 0.6%, strawberry mango at 0.4%, rainbow candy at 0.4%, strawberry ice cream at 0.4%, marshmallow at 0.3%, apple ice at 0.2%, refreshing at 0.2%, strawberry banana at 0.2%, red bomb at 0.2%, chilled berry lemon at 0.2%, summer strawberry kiwi at 0.1%, aloe grape at 0.1%, tropical fruit Hawaii at 0.1%, rum ice at 0.1%, strawberry watermelon at 0.1%, banana ice at 0.1%, and others at 0.4%

After centrifugation at 1500 rpm for 5 min, the cell pellet was resuspended in 300 L PBS, and 700 L pre-cooled anhydrous ethanol was slowly added dropwise while the tube was stored at 4 C for overnight fixation

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