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cDNA synthesis and quantitative real-time PCR were subsequently performed using the PrimeScript RT Reagent Kit and SYBR Premix Ex Taq II (Thermo Fisher Scientific, USA) under the following cycling conditions: 95 C for 5 min

For example, ES reduced synaptophysin (SYP) and post-synaptic density (PSD)-95 expression, important in the formation and maintenance of synapses and their transmission [174, 175]

Results vary based on factors such as dosage, frequency of administration, and individual response

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