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A 10 ml sample of each culture was harvested by centrifugation at 5000 g for 10 min and resuspended in 4 ml of lysis buffer (50 mM NaH 2 PO 4 /Na 2 HPO 4 , 0.3 M NaCl, 20 mM imidazole, pH 8.0), then lysed by sonication on ice

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And a 2006 review of the existing research by the respected Cochrane organization found that while no studies unequivocally demonstrated the effectiveness of AA or [12-step facilitation treatment] approaches for reducing alcohol dependence or problems, 12-step treatment fared about as well as other treatment programs